Public perception of deceased organ donation in Trinidad

Objective: To investigate person's knowledge, attitude and practice (KAP) toward Deceased Organ Donation (DOD) in Trinidad. Design and Methodology: A cross-sectional study of patients attending four randomly selected health centres in Trinidad was conducted using a using quota sampling, to obtain equal numbers of males and females. Data was collected using a 41-item questionnaire, which was structured to test knowledge, attitude and practices. Chis-sq analysis was conducted using SPSS Version-23. Results: 273 respondents completed the questionnaire; 50.5% females; mean age 39.5 (SD15.1) years; 50.2% Indo-Caribbean and 24.5% African, 47.8% completed secondary education; and majority Christian (48.4%) or Hindu (31.5%). 81.3% had heard of the term organ donation". Respondents believed the most frequently reported organs for deceased organ transplant in Trinidad were kidney (76.1%), heart (48.2%) and liver (33.3%). When asked about their attitudes toward DOD, 52.3% of the respondents stated that they would donate their organs, 79% had not heard about the National Organ Transplant Unit (NOTU) and no respondent reported ever donating an organ, nor enquired about donor cards (80%), nor heard about support groups (68%). Conclusion: Most participants, though knowledgeable on the term DOD, were not familiar with the practice nor the major organ donation groups in Trinidad such as NOTU and the Kidney Recipients Support Group. However, participants were positive toward DOD, thus, raising greater awareness can lead to major cost-benefits for the public health system.

Increased levels of brain serotonin correlated with MMP-9 activity and IL-4 levels resulted in severe experimental autoimmune encephalomyelitis (EAE) in obese mice.

The aim of this study was to investigate the role of monoamine neurotransmitters on the severity of experimental autoimmune encephalomyelitis (EAE) in obese mice. EAE was induced in mice with normal diets (ND-EAE) and obese mice with high-fat diets (HFD-EAE) through the immune response to myelin oligodendrocyte glycoprotein (MOG) (35-55). The levels of dopamine (DA), serotonin (5-HT) and their metabolites in different anatomical brain regions were measured by high-performance liquid chromatography. The plasma and tissue NADPH oxidase and matrix metalloproteinases (MMP)-9 activities were analyzed by fluorescence spectrophotometry. The cumulative disease index and disease peaks were significantly higher in HFD-EAE compared with those in ND-EAE. Significantly higher 5-HT levels and lower 5-HT turnovers 5-hydroxyindole acetic acid ((5-HIAA)/5-HT) were found in the brains of HFD-EAE mice compared with those found in the HFD-CON and ND-EAE mice brains. Moreover, increased DA levels were observed in the caudate nucleus of the HFD-EAE mice compared with the control and ND-EAE mice. The NADPH oxidase and MMP-9 activities in the plasma and tissues were significantly higher in both the ND-EAE and HFD-EAE groups than in their respective controls. The cytokine levels in the plasma, tissues, and cultured splenocytes were found to be significantly altered in EAE mice compared with control mice. Moreover, HFD-EAE mice exhibited significantly higher MMP-9 activity and lower IL-4 levels than ND-EAE mice and were significantly correlated with brain 5-HT levels. In conclusion, the increased 5-HT levels in the brain significantly correlated with MMP-9 activity and IL-4 levels play an important role in the exacerbation of disease severity in HFD-EAE mice.